Picture of                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     Samantha Ekanayake

Samantha Ekanayake Technician, Research and Graduate Studies, WCVM

Profile

Samantha Ekanayake provides technical support and training to clinical research in following areas:  

  • Virological methods
  • Cell culture techniques, cell signaling
  • Field research – sample and data collection, veterinary clinical procedures
  • Bacteriological methods
  • Patasitological methods
  • Molecular techniques - Conventional and multiplex PCR, real-time qPCR (SYBR Green, Taq-Man), reverse-transcript PCR, Sanger sequence analysis using  Staden Package, NCBI BLAST, primer design, gene selection
  • Florescent in situ hybridization (FISH)
  • Agarose gel electrophoresis, SDS-PAGE, Western blot
  • ELISA development
  • Histological techniques, immunohistochemistry, apoptosis assay
  • Bight field microscopy, fluorescence microscopy
  • Animal experimentation, necropsy procedures, rodent research
  • User/assist animal nuclear imaging with PET-CT Scanner at Saskatchewan Centre for Cyclotron Sciences
  • Research communication, visual presentation

Academic Credentials

  • BVSc, University of Peradeniya — 1994
  • MPhil, University of Peradeniya — 2005
  • MSc, University of Saskatchewan — 2009

Publications

Ek, C.E., Nosach, R., Fernando, C., Huang, Y., Perez, J.B.D.S., Costa, M.O., Ekanayake, S. Hill, J.E. and Harding, J.C.S. (2017). An optimized murine model to characterize shedding and clinical disease associated with “Brachyspira hampsonii” infection. BMC Veterinary Research: 13: 261-272.  doi:  10.1186/s12917-017-1166-5

Predicala, B., Alvarado, A. and Ekanayake, S. (2015). Field testing of an air filtration system for a pig transport trailer. Annual Research Report 2013-14, Prairie Swine Centre Inc., 25-26

Predicala, B., Alvarado, A. and Ekanayake, S. (2014). Field Testing of an air filtration system for a transport trailer. Western Hog Journal, Summer, 52-54

Rubin, J. E., Ekanayake, S. and Fernando , C(2014). Pseudomonas sp. AM05CRO1 VIM-2 type carbapenemase gene, partial cds. GenBank Accession No. KJ625238 https://www.ncbi.nlm.nih.gov/nuccore/kj625238

Rubin, J. E., Ekanayake, S. and Fernando, C. (2014). Pseudomonas sp. AM05CR01 chaperonin 60 (cpn60) gene, partial cds, GenBank Accession No. KJ606641.1, https://www.ncbi.nlm.nih.gov/nuccore/KJ606641.1

Rubin, J. E., Ekanayake, S. and Fernando, C. (2014). Carbapenamase-producing organism in food, 2014. Emerging Infectious Diseases, 20(7): 1264-1265 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4073846/