Picture of                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    Ali Honaramooz

Ali Honaramooz Professor, Department of Veterinary Biomedical Sciences

Address
WCVM 2255

Research Area(s)

  • Reproductive biology and technology

Academic Credentials

  • DVM, Shiraz University
  • PhD, University of Saskatchewan
  • Post-doctoral training, University of Saskatchewan and University of Pennsylvania

Teaching and Clinical Areas

Dr. Honaramooz's teaching include participation in team-taught courses in the veterinary (DVM) program:

  • VBMS 220.8 (Veterinary Anatomy; course coordinator)
  • VBMS 224.9 (Veterinary Physiology)
  • VBMS 208.1 (Biomedical Rounds)

 He also teaches in the graduate student program:

  • VBMS 830.3 (Physiology and Endocrinology of Reproduction; course coordinator)
  • VBMS 898.3 (Models of Testis Function; course coordinator)

Research Interests

Dr. Honaramooz's research interests are in reproductive biology and technology, with emphasis on the study and manipulation of spermatogenesis and spermatogonial stem cells. His research team has developed the technique for germ cell (spermatogonial) transplantation in farm animals, a procedure in which testis cells are harvested from a fertile donor male and microinjected into the seminiferous tubules of an infertile recipient. This system provides a unique functional assay to determine stem cell potential of a given population of donor germ cells.

Studies are underway to improve the outcome of germ cell transplantation to be used as a viable alternative approach to generate transgenic farm animals. Honaramooz and his team members have also established the technique for testis tissue xenografting, a model system that allows the progression of spermatogenesis from a variety of mammalian species in a laboratory mouse.

Using this system, it has now become feasible to produce sperm even from newborn domestic and wild animals in a host mouse. Testis tissue xenografting has opened a new avenue of research in reproductive biology, and the Honaramooz research team's current studies are aimed at introducing this technology as a novel tool for

  • the study and manipulation of spermatogenesis in different species
  • the conservation of valuable immature individuals such as endangered species and prized farm animals
  • the potential preservation of fertility in prepubertal boy cancer patients undergoing gonadotoxic treatments

Dr. Honaramooz’s research has also led to the introduction of a system for “germ cell transplantation” in farm animals, a procedure in which testis cells are harvested from a fertile donor male and microinjected into the seminiferous tubules of an infertile recipient. This enables the recipient to become fertile and pass on the donor-derived or genetically-modified traits into progeny, and resulted in the production of world’s first transgenic farm animals using this new technology. This innovative system can potentially be a faster, more efficient, and less-costly approach than cloning for production of transgenic farm animals. Dr. Honaramooz’s lab also conducts focused on:

  • the study of male germline stem cells both in-vitro and in-vivo
  • using germ cell transplantation as a bioassay for stem cell potential of a given germ cell population
  • improving the outcome of germ cell transplantation as a viable option for generation of transgenic farm animals

Dr. Honaramooz is also a member of the One Reproductive Health team of researchers at the University of Saskatchewan (http://www.usask.ca/groups/onereproductivehealth/). If you would like to join Dr. Honaramooz’s research team, please contact Dr. Ali Honaramooz by email: ali.honaramooz@usask.ca.

Publications

Select Recent Publications (trainees underlined):

Olubamiji A.D, Zhu N, Chang T, Izadifar Z, Nwankwo C, Honaramooz A, Chen XB, Eames BF. 2017. “Traditional invasive and synchrotron-based non-invasive assessments of 3D-printed hybrid cartilage constructs.” Tissue Engineering, Part C. In Press.

Villagómez DAF, Revay T, Donaldson B, Rezaei S, Pinton A, Palomino M, Junaidi AWA, Honaramooz A, King WA. 2016. “Azoospermia and testicular hypoplasia in a boar carrier of a novel Y-autosome translocation.” Sexual Development. DOI: 10.1159/000453298. In Press.

Izadifar Z, Honaramooz A, Wiebe S, Belev G, Chen X, Chapman D. 2016. “Low-dose phase-based X-ray imaging techniques for in situ soft tissue engineering assessments.” Biomaterials. 82:151-67. doi: 10.1016/j.biomaterials.2015.11.044.

Chen J, Yang Y, Abbasi S, Hajinezhad D, Kontulainen S, Honaramooz A. 2015. “The effects of elk velvet antler dietary supplementation on physical growth and bone development in growing rats.” Evidence-Based Complementary & Alternative Medicine. 2015: 819520, doi:10.1155/2015/819520.

Honaramooz A. 2014. “Potential and challenges of testis tissue xenografting from diverse ruminant species.” In: Reproduction in Domestic Ruminants VIII. J.L. Juengel, A. Miyamoto, C. Price, L.P. Reynolds, M.F. Smith and R. Webb (eds.). Pages: 257-275. Leicestershire, England: Context Products Ltd. (ISBN: 978-1899-04-3637).

Mankidy R, Ranjan B, Honaramooz A, Giesy JP. 2014. “Effects of novel brominated flame retardants on steroidogenesis in primary porcine testicular cells.” Toxicology Letters. 224(1): 141-146. 

Zeng W, Tang L, Bondareva A, Honaramooz A, Tanco V, Dores C, Megee S, Modelski M, Rodriguez-Sosa JR, Paczkowski M, Silva E, Wheeler M, Krisher RL, Dobrinski I. 2013. “Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs.” Biology of Reproduction. 88(1): 27, 1-9.

Abbasi S, Honaramooz A. 2012. “Feasibility of salvaging genetic potential of post-mortem fawns: Production of sperm in testis tissue xenografts from immature donor white-tailed deer (Odocoileus virginianus) in recipient mice.” Animal Reproduction Science. 135(1-4): 47-52.

Yang Y, Honaramooz A. 2012. “Characterization and quenching of autofluorescence in piglet testis tissue and cells.” Anatomy Research International. 2012: 820120.                

Chen J, Woodbury MR, Alcorn J, Honaramooz A. 2012. “Dietary supplementation of female rats with elk velvet antler improves physical and neurological development of offspring.” Evidence Based Complementary & Alternative Medicine. 2012: 640680.

Honaramooz A. 2012. “Cryopreservation of testicular tissue.” In: Current Frontiers in Cryobiology. I. Katkov (Ed.). Pages: 209-228. Rijeka: InTech Publication. (ISBN: 978-953-51-0191-8).

Honaramooz A, Yang Y. 2011. "Recent advances in application of male germ cell transplantation in farm animals." Veterinary Medicine International. Doi; 10.4061/2011/657860.

Abbasi SHonaramooz A. 2011. "Xenografting of testis tissue from bison calf donors into recipient mice as a strategy for salvaging genetic material." Theriogenology. 76: 607-614.

Yang YHonaramooz A. 2011. "Efficient purification of neonatal porcine gonocytes with Nycodenz and differential plating." Reproduction, Fertility and Development. 23: 496-505.

Zeng W, Baumann C, Schmidtmann A, Honaramooz A, Tang L, Bondareva A, Dores C, Fan T, Xi S, Geiman T, Rathi R, de Rooij D, De La Fuente R, Muegge K, Dobrinski I. 2011. "Lymphoid-specific helicase (HELLS) is essential for meiotic progression in mouse spermatocytes." Biology of Reproduction. 84: 1235-1241.

Abbasi SHonaramooz A. 2011. "The number of grafted fragments affects the outcome of testis tissue xenografting from piglets into recipient mice." Veterinary Medicine International. Doi: 10.4061/2011/6686570.

Yang Y, Yarahmadi MHonaramooz A. 2010. "Development of novel strategies for the isolation of piglet testis cells with a high proportion of gonocytes." Reproduction, Fertility and Development. 22: 1057-1065.

Abbasi SHonaramooz A. 2010. "The effects of recipient mouse strain, gender and gonadal status on the outcome of testis tissue xenografting." Reproduction, Fertility and Development. 22: 1279-1286.

Turner R, Rathi R, Honaramooz A, Zeng W, Dobrinski I. 2010. "Xenografting restores spermatogenesis to cryptorchid testicular tissue but does not rescue the phenotype of idiopathic testicular degeneration in the horse (Equus caballus)." Reproduction, Fertility and Development. 22(4): 673-683.

Yang Y, Steeg JHonaramooz A. 2010. "The effects of tissue sample size and media on short-term hypothermic preservation of porcine testis tissue." Cell and Tissue Research. 340(2): 397-406.

Yang YHonaramooz A. 2010. "Effects of medium and hypothermic temperatures on preservation of isolated porcine testis cells." Reproduction, Fertility and Development. 22(3): 523-532.

Abrishami M, Abbasi SHonaramooz A. 2010. "The effect of donor age on progression of spermatogenesis in canine testicular tissue after xenografting into immunodeficient mice." Theriogenology. 73(4): 512-522.

Abrishami M, Anzar M, Yang Y, Honaramooz A. 2010. "Cryopreservation of immature porcine testis tissue to maintain its developmental potential after xenografting into recipient mice." Theriogenology. 73(1): 86-96.

Honaramooz A, Li MW, Penedo CT, Meyers SA, Dobrinski I. 2004. "Accelerated maturation of primate testis by xenografting into mice." Biology of Reproduction. 70: 1500-1503.

Honaramooz A, Behboodi E, Megee SO, Overton SA, Galantino-Homer H, Echerlard Y. Dobrinski I. 2003. "Fertility and germline transmission of donor haplotype following germ cell transplantation in immuno-competent goats." Biology of Reproduction. 69: 1260-1264.

Honaramooz A, Snedaker A, Boiani M, Schöler H, Dobrinski I, Schlatt S. 2002. "Sperm form neonatal mammalian testes grafted in mice." Nature. 418: 778-781.

Honaramooz A, Megee SO, Dobrinski I. 2002. "Germ cell transplantation in pigs." Biology of Reproduction. 66: 21-28.

For a full list of publications please see (https://scholar.google.ca/citations?user=90v2pIIAAAAJ&hl=en&oi=ao.).

 

s." Biology of Reproduction. 66: 21-28.