- Use of the bovine model of maternal reproductive aging for the study of ovarian function in humans
- BVSc and AH (1983, Punjab Agril. University of India)
- MVSc (1986, Punjab Agril. University of India)
- PhD (1997, University of Saskatchewan, Canada)
Dr. Singh's teaching expertise is in the area of microscopic anatomy and reproductive science with special interest in ovarian follicle and oocyte development, and early embryonic development. He has taught microscopic anatomy to first year veterinary students at the Western College of Veterinary Medicine since 1999. His graduate teaching includes physiology and endocrinology of reproduction in mammals, techniques in reproduction, and ultrastructural cytology. He has co-directed nine continuing-education reproductive ultrasonography and embryo transfer training workshops during his academic career. He has supervised or co-supervised 13 MSc, 8 Ph.D. and more than 40 undergraduate students, and has hosted 3 post-doctoral fellows and 9 faculty members from around the World.
Dr Singh’s research program is focused on factors that influence an oocyte’s ability to develop into an embryo during first 7 days of life (i.e., oocyte competence). Over the past decade, his research team has established and validated the bovine model of maternal reproductive aging for the study of ovarian function in humans. His current and past research has been directed towards studying the ultrasonographic, morphologic and biochemical kinetics and endocrine control of ovarian follicles. He focuses on to investigate the developmental competence of oocytes contained in these follicles for refining controlled breeding programs (e.g. fixed-time artificial insemination and superovulation procedures) to improve reproductive efficiency in beef cattle and to help with assisted reproduction in humans. His particular interest is in direct in vivo imaging of cumulus oocyte complexes using ultrasound biomicroscopy, echotexture analysis, and 3D visualization of cellular organelles in oocytes. Recently, his research group has examined the role of alterations in granulosa cell transcriptome during follicular and maternal aging. His research program has been funded by the discovery grants from the Natural Sciences and Engineering Research Council of Canada for the past 15 years and by support from the Canadian Foundation for Innovation, Canadian Light Source, EmbryoGENE Network, Prostate Canada, Sylvia Fedoruk Centre for Nuclear Innovation, Saskatchewan Agriculture Development Fund, Saskatchewan Cattlemen’s Association, and Saskatchewan Health Research Foundation. He has published more than 100 original research articles or review papers in scientific journals, 175 abstracts and presented more than 20 Invited talks. His current research projects extend from the study the basic molecular, cellular and endocrine control of ovarian follicles (neuroendocrine control pathways in the bovine hypothalamus, follicle development and oocyte competence during pre- and peripubertal period) to field experiments for optimizing reproductive efficiency and health in cattle (e.g., 7-day FSH protocol for superovulation; effect low-levels of ergot alkaloid in animal feed on reproductive performance of beef cows and breeding bulls) and to the development of domestic animal models for the study of human disorders (canine models of benign prostatic hyperplasia and prostate cancer in men, and endometriosis in women). His research group maintains the Oocyte Competence Laboratory and the Molecular and Live-cell imaging facility at the Western College of Veterinary Medicine.
Adams GP, Singh J (2015) Ovarian follicular and luteal dynamics in cattle (Book Chapter) In Bovine Reproduction Ed. Hopper RM, pp 219-244 first Edn. John Wiley & Sons Inc. IOWA USA
Dias FCF, Khan MIR, Adams GP, Sirard MA, Singh J (2014) Granulosa cell function and oocyte competence: Super-follicles, super-moms and super-stimulation in cattle Animal Reproduction Science 149: 80-89.
Dadarwal D, Honparkhe M, Dias FCF, Alce T, Lessard C, Singh J (2014) Effect of superstimulation protocols on nuclear maturation and distribution of lipid droplets in bovine oocytes Reproduction, Fertility and Development doi.10.1071/RD13265
Dias FCF, Khan MI, Sirard MA, Adams GP, Singh J (2013) Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle Reproduction 146: 181–191
Malhi PS, Adams G and Singh J (2005) Bovine Model for the Study of Reproductive Aging in Women: Follicular, Luteal and Endocrine Characteristics Biology of Reproduction 73: 45-53.
Singh J, Pierson R A and Adams G P (1997) Ultrasound image attributes of the bovine corpus luteum: structural and functional correlates Journal of Reproduction and Fertility 109: 35-44.