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Western College of Veterinary Medicine

About

The Olympus IX83 inverted microscope is equipped with a TIRF system, capable of high resolution imaging near the coverslip interface eliminating out of focus signals.

This system also offers epifluorescence imaging and an environmental chamber for live cell imaging (short-term or long-term imaging, such as one week).   

Features and specifications

  • TIRF with 405, 488, 561 and 640 nm laser excitations  
  • A wide-field fluorescent microscope with 365, 460, 565 and 635 nm LED excitations 
  • Highly sensitive and fast frame rate cameras (iXon Ultra EMCCD and Zyla sCMOS) and a high-performance colour camera (DP73) 
  • Bright field, DIC, phase contrast and polarized microscopy 
  • Equipped with a humidified stage top incubator with temperature and CO2 controls 
  • Z-drift compensation keeps the sample always in focus, advantageous for automated live cell imaging 
  • Capable of fully automated stitching of fluorescent images 
  • Comes with cellSens image analysis software capable of deconvolution, high-content and conventional image analyses 

Imaging examples

Comparison of TIRF and epifluorescenceimaging. A placenta cell immunolabelledfor focal adhesion proteins. Courtesy of Dr. Daniel MacPhee, WCVM

 

fluoresence imaging
This image covering an area of approx. 1 cm2(left) was created by automated stitching of over 700 individual fluorescent images. An area marked in orange rectangle is zoomed in to show details (right). Sample: Fluorescent brain section. Courtesy of Carlos Leonardi and Dr. Jaswant Singh, WCVM. 

  

 

TIRF live-cell imaging of fluorescently tagged EB1, which labels growing microtubule ends, in Arabidopsis.
Courtesy of Dr. Chris Ambrose, Department of Biology, USask College of Arts and Science. 

Booking

Booking use of the Olympus IX83 inverted microscope

To ensure the availability of the Olympus IX83 inverted microscope, please book the time that you are intending to use the equipment in advance.

To view the microscope's availability, please visit the multi-user calendar. Please note: you must be a University of Saskatchewan PAWS user with a valid NSID and you must be logged in to PAWS before you can book times on the following calendar.

To book equipment: please click on the selected date and select the "Add+" symbol in the bottom corner of the date box to open the calendar submission form. Please fill in all of the form's fields. Remember to include your name and telephone number as well as your start time and end time in the form's title box. 

To cancel your equipment booking: please click on the event to open an "Edit" window. Click "Delete" on the form. 

For more information about arranging use of the above equipment, please contact the WCVM Imaging Centre.