Scanner systems
Li-Cor Odyssey Clx imaging system
A near infrared (NIR) fluorescence digital detection system used for Western blots, cell-based assays, protein arrays, gel shift assays and tissue section imaging, using IRDye 700 nm (red) and IRDye 800 nm (green) in order to multiplex.
It has a large scanning bed, which can accommodate high throughput for many assays.
The Li-Cor Odyssey boasts great sensitivity and no image saturation.
Powerful, precise laser excitation and specialized optics enable the Odyssey CLx to provide high signal-to-noise ratios and outstanding image quality and unprecedented linear dynamic range.
Our instrument is paired with Image Studio Software for streamlined image acquisition and organization and with new Empiria Studio Software for analysis of your Western blots.
To ensure the availability of the Li-Cor Odyssey Clx imaging system, please book the time that you are intending to use the equipment in advance. To view the Li-Cor Odyssey Clx imaging system's availability, please visit the multi-user calendar. Please note: you must be a University of Saskatchewan PAWS user with a valid NSID and you must be logged in to PAWS before you can book times on the following calendar.
To book equipment: please click on the selected date and select the "Add+" symbol in the bottom corner of the date box to open the calendar submission form. Please fill in all of the form's fields. Remember to include your name and telephone number as well as your start time and end time in the form's title box.
To cancel your equipment booking: please click on the event to open an "Edit" window. Click "Delete" on the form.
For more information about arranging use of the above equipment, please contact Champika Fernando (306-966-7228), WCVM molecular microbiology lab co-ordinator.
Typhoon scanner system
The Typhoon instrument is a variable-mode imager that produces digital images of radioactive, fluorescent or chemiluminescent samples. Our Typhoon is equipped with three lasers to illuminate samples. They are the red laser (633 nm), the green laser (532 nm) and the blue laser (488 nm). Along with this, the Typhoon contains a standard set of emission filters.
After the scan, you use the ImageQuant software to visualize the digital information and produce an accurate image of the original sample. In ImageQuant you can view a set of images overlaid in different colors (multilabel experiments) or you can view the channels side by side in gray scale. Results can by analyzed qualitatively and quantitatively in ImageQuant. Quantitation is possible because the Typhoon system provides a linear response to the fluorescent signal intensities.
It is not unusual to spend one hour or more refining your scan to get it looking as good as possible, and therefore, it should be booked for your use ahead of time.
The Typhoon scanner has been relocated to the USask Department of Chemistry, Thorvaldson Building. Please contact Christopher Phenix (chris.phenix@usask.ca) to arrange use of the equipment.